FastQCFastQC Report
Sun 14 Apr 2019
SRR6576675_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576675_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences287498
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7070.24591475418959435No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3710.12904437596087626No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3450.12000083478841592No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3310.11513123569555266No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3260.11339209316238721No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3140.10921815108279015No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCCGC757.422739E-637.6010441
GTATCAA8450.037.2673071
TAGACTA550.002717899434.1708765
GTACATG16800.033.852131
TACATGG16700.033.2105032
ATTATAC851.7540662E-533.165853
ACATGGG17050.032.2418753
CATGGGT4650.031.3233014
ACTATAC1052.0633997E-631.3233013
ATCAACG9900.030.3741113
AACGCAG10100.030.237846
TACTATA1102.9576058E-629.9099222
TCAACGC10200.029.4807554
CCAATAC650.006156219628.9138183
ATAAGTG650.006156219628.9138185
ATGGGTA2950.028.6687835
CAACGCA10750.028.4095085
TGGGTAC3150.028.3401326
CATGGGG6000.027.4078884
TATACAG1904.456524E-1027.2018155
Produced by FastQC (version 0.11.2)