FastQCFastQC Report
Sun 14 Apr 2019
SRR6576675_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576675_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences287498
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5420.18852305059513458No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3910.13600094609353805No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3470.1206964918016821No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3160.10991380809605633No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3120.10852249406952397No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3120.10852249406952397No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3060.10643552302972542No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG17650.036.248041
ACATGGG17900.035.4419443
TACATGG17800.035.38322
TATACTA702.1805156E-433.5725482
GTATCAA9750.033.291611
CATGGGA8750.031.6869724
AACGCAG10600.029.7033676
ATATAGC804.776E-429.3708696
ATCAACG11250.028.4048923
CAACGCA11600.027.5478485
TCAACGC11800.027.0809384
CCCATAT700.008786377526.8814241
CATGGGG6150.026.7442054
CATGGGT4700.025.9963444
ACGCAGA12450.025.2896147
CGCAGAG12800.024.6152328
TATCAAC13150.024.3049932
ATGGGAT2602.7284841E-1123.4966955
ATGGGAG3800.023.4966955
GGGTACC2653.6379788E-1123.053367
Produced by FastQC (version 0.11.2)