FastQCFastQC Report
Sun 14 Apr 2019
SRR6576676_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576676_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences448383
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11460.2555850690146593No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT7140.1592388649881909No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7050.15723165240430614No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA6030.13448324312027887No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5960.1329220777772574No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4730.10549017246416569No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4720.10526714884373403No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4710.1050441252233024No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4690.10459807798243913No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4580.10214481815769107No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG4570.10192179453725944No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12900.037.5347021
TACATGG28000.035.257112
GTACATG28600.034.8461881
ACATGGG28700.034.3895073
TACGGTG702.180301E-433.5781972
ATGGGTA4600.031.6732395
GGTACCT4350.031.3326638
AACGCAG16100.031.2353576
GGGTACC4450.030.628567
TGGGTAC4550.029.9554046
CATGGGG10200.029.9503384
ATCAACG16250.029.7901333
CATGGGT6950.029.754764
CAACGCA17150.029.322995
TCAACGC17050.028.6680094
ATGGGCG1354.6249443E-727.8512575
GCAAACG856.8015343E-427.6526361
TACACCG700.008831326.856575
ACGCAGA18800.026.749437
CATGGGA14550.026.4874064