FastQCFastQC Report
Sun 14 Apr 2019
SRR6576678_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576678_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences327690
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5170.15777106411547498No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3550.1083340962495041No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10900.041.836081
ACTCGTC450.001013176941.7754028
GTACATG23700.037.4903261
TACATGG23650.036.9732442
ACATGGG24200.035.927713
AACGCAG13750.032.812686
CATGGGT5050.032.572414
ATCAACG14000.032.2267383
GTATAGC1052.0554235E-631.3411181
TCAACGC14400.031.3315534
CAACGCA14200.031.110915
CATGGGG8050.030.942344
CATGGGA13600.029.71894
TATCAAC15500.029.116912
GTCTAGA1154.1762796E-628.6158031
ACGCAGA16050.027.5249167
CGCAGAG16450.026.855628
ATGGGAG5600.026.8556185
CTAATAG909.5062656E-426.1096273
TATAGCC1501.1557859E-625.0728952
Produced by FastQC (version 0.11.2)