FastQCFastQC Report
Sun 14 Apr 2019
SRR6576679_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576679_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences302543
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5860.19369147526136782No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4830.15964672790314105No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3410.11271125096267308No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG21100.037.691361
CTAGGGT500.001700720437.596964
CATGGGT4150.037.370474
GTATCAA13050.037.1419141
TACATGG21450.037.027312
TAGTACC651.4117E-436.150924
ACATGGG21950.035.9697573
GTATTGG801.14602735E-535.293831
GGGTACC2500.033.837267
ATGGGTA3350.032.2660455
GGTACCT2650.031.9377888
AACGCAG15800.030.636766
ATCAACG16200.030.1703993
CATGGGG7250.029.8182754
CTATGCT804.7749138E-429.3726224
CAACGCA16800.029.0928825
TCAACGC16850.029.0065544
GTTATAT1005.2659423E-528.2350671
ACGCAGA17450.027.4705547
TATCAAC18050.027.3385072
Produced by FastQC (version 0.11.2)