FastQCFastQC Report
Sun 14 Apr 2019
SRR6576681_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576681_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences340530
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9630.28279446744780196No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6380.18735500543270783No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5400.1585763368866179No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5030.14771092121105336No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4930.14477432237981971No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4350.12774204915866444No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4330.1271547293924177No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4130.12128153172995036No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3980.11687663348309987No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3840.11276539511937275No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3600.10571755792441195No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3590.10542389804128857No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT3590.10542389804128857No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3560.10454291839191848No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG3430.10072533991131472No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10500.043.885111
GTACATG22250.042.8989261
TACATGG22850.042.389812
ACATGGG22950.041.367353
TATACGG500.001696837737.615812
TACACGC500.00170051537.599246
AACGCAG12650.036.4103286
CATGGGA12150.036.36144
ATCAACG12550.036.325963
TCAACGC12800.035.6164634
CAACGCA13400.034.3724375
ACGCAGA14150.032.550587
CGCAGAG14500.031.7648728
CATGGGG8600.030.6040294
TGGGTAC3400.030.4111466
TATCAAC15850.029.3688092
CATGGGT5450.029.3205054
ATAATGC650.00614877928.9224913
ATGGGTA3850.028.0773535
CATGGGC3900.027.7173864
Produced by FastQC (version 0.11.2)