FastQCFastQC Report
Sun 14 Apr 2019
SRR6576682_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576682_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences307544
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8550.278009000338163No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5750.1869651171864839No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5150.1674557136539812No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5120.16648024347735607No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4530.14729599667039514No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4430.14404442941497803No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3990.12973753349114273No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3990.12973753349114273No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3980.12941237676560102No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3700.1203079884504331No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3670.11933251827380797No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3420.1112036001352652No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG3410.1108784434097235No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT3350.10892750305647322No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC3100.10079858491793045No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG22850.039.6869741
TACATGG23500.038.589252
ACATGGG23550.038.108283
GTATCAA9950.037.778421
ATGGGTA3000.037.589535
CATGGGT5350.035.13044
GTATAAC1107.683957E-834.17231
TTAGGGT702.1848678E-433.562084
CATGGGG8950.031.4996034
AACGCAG11750.030.7914226
ATGGGAG4500.030.2804535
TGGGTAC3450.029.9626676
CATGGGC3300.028.4769154
GGTACCT3650.028.3208758
GGGTACC3650.028.3208757
ATCAACG12750.028.0078833
CAACGCA13000.027.830715
TCAACGC12850.027.7899254
CCATCGT856.8157475E-427.6393694
ATGGGGA3600.027.4090335
Produced by FastQC (version 0.11.2)