FastQCFastQC Report
Sun 14 Apr 2019
SRR6576682_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576682_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences307544
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8060.2620763207866192No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5440.17688525869469085No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5060.16452930312410582No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4650.15119787737689566No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4550.14794631012147855No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4350.14144317561064434No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4130.13428972764872668No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3960.1287620633145176No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3810.12388471243139193No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3500.11380485393959888No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3470.11282938376297376No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3410.1108784434097235No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3350.10892750305647322No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3340.10860234633093152No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT3200.10405015217334755No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG3160.1027495252711807No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGCG200.00215702270.53660694
CCGTCTA250.005221660756.4292879
TACTAGG952.1107553E-839.5737462
GTATCAA10250.038.10311
GTACATG22800.037.974231
GTACTAG1003.279456E-837.6440281
TACATGG23250.037.595062
ACATGGG23100.036.8223
TACTATC651.4121755E-436.149097
CATGGGG7300.032.187554
TTACTAC600.00415896331.3292123
GGGTTAG600.00415896331.3292127
CATGGGA11500.030.6481444
ATGGGAG5400.030.458965
ATGGGTA3850.029.294855
AATAGGC650.00615120628.9192733
AACGCAG13800.027.9238646
GGTACCT3200.027.920748
TAATAGG856.8098906E-427.6434252
CATGGGT6400.027.1683034
Produced by FastQC (version 0.11.2)