FastQCFastQC Report
Sun 14 Apr 2019
SRR6576683_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576683_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences438856
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9570.21806697413274514No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6720.15312539876405928No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5610.12783236414678165No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5330.12145213919827916No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4890.11142607142206098No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4700.1070966330641486No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4520.10299505988296843No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12050.048.374761
AACGCAG15400.037.8430566
ATCAACG15350.037.660143
TCAACGC15750.037.0020984
CAACGCA15850.036.7686465
ACATGGG26750.036.0176433
CATGGGT5500.035.8898664
GTACATG27400.035.8574941
TACATGG27200.035.7754972
TAATACC702.183183E-433.5704544
ACGCAGA17900.032.5577137
CGCAGAG17850.032.3856128
TATCAAC18650.032.0117262
ATAATAC1602.1263986E-929.3741463
CATGGGG10050.028.9941834
CATGGGA14050.028.4333364
ATGGGAG6550.027.983925
GCAGAGT20800.027.7924619
GTGTAAC856.8014156E-427.6525571
ATGGGTG2500.026.3192375
Produced by FastQC (version 0.11.2)