FastQCFastQC Report
Sun 14 Apr 2019
SRR6576684_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576684_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences394142
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8270.20982285572204942No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5980.15172196822465No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5650.14334935124904222No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5510.13979733192605712No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5130.1301561366208118No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4590.11645549066072634No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4180.10605314835769848No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4020.10199369770285836No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3990.10123255070507582No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCGT250.005236822656.390946
GTATCAA12450.041.6038861
GTACATG25650.033.962131
TACATGG25850.033.4534152
ACATGGG26000.033.0754553
ATCAACG15550.032.3356443
AACGCAG15650.032.1290256
TCAACGC15850.031.723614
CAACGCA16200.031.0382235
GTATGGG1102.9656348E-629.9042873
CATGGGT6450.028.4140424
CGCAGAG17750.028.3494248
ATGGGTA4350.028.0874425
ACGCAGA17900.027.827937
TATCAAC18500.027.436912
CATGGGG8750.027.3898874
TGGGTAC4050.026.687076
GGGTACC4050.026.687077
CATGGGA14700.026.2134744
ATGGGAT4600.025.5393755
Produced by FastQC (version 0.11.2)