FastQCFastQC Report
Sun 14 Apr 2019
SRR6576686_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576686_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences233896
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7110.3039812566268769No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3900.16674077367718987No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3730.1594725861066457No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3700.15818996477066732No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3500.14963915586414475No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3290.14066080651229607No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3090.13210999760577352No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2950.1261244313712077No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG2750.11757362246468517No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2690.1150083797927284No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT2670.11415329890207614No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC2600.11116051578479323No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2550.1090228135581626No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC2450.10474740910490132No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2420.10346478776892293No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAACT301.3666699E-462.6872061
GTAATAC601.603079E-646.985253
TACGACG450.001013862741.7646685
CGACGGT500.001701879237.5881967
TACATGG16350.035.081832
ACATGGG15900.034.8695533
TGGGTAC1900.034.620716
GTACATG16300.034.6125681
CATGGGT3150.032.8150984
CATGGGC3400.031.7841384
CATGGGG5850.031.3234984
CCGGTCC753.2736524E-431.3234989
ACGACGG600.004160657531.3234986
ATGGGAG2700.031.3234965
GTATCAA10950.030.914241
ATGGGTA2300.030.6425535
AACGCAG11200.030.6243156
GGGTACC2150.030.5950437
ATGGGGC2250.029.2352645
GTCCTAT1005.2938252E-528.2092421
Produced by FastQC (version 0.11.2)