FastQCFastQC Report
Sun 14 Apr 2019
SRR6576688_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576688_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences266191
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6260.2351694835663114No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3920.14726267980510235No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3820.14350597878966606No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3380.12697649432174643No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3080.11570639127543757No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2740.1029336078229542No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2730.10255793772141056No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10000.041.8845061
ATGGGAC2200.040.5827145
GTACATG19150.039.565851
TACATGG18950.039.4273952
ACATGGG19250.038.3247263
CATGGGA10200.036.8553054
TCTATAC1055.0958988E-835.8022963
TATACTG1204.1891326E-935.242885
ATCAACG11650.035.091633
AACGCAG11800.034.2473226
TCAACGC12050.033.926764
CAACGCA12300.033.237195
TATCAAC13450.031.4434682
CATGGGG6000.031.3270074
CGCAGAG13000.030.7476718
ACGCAGA13050.030.6068487
GTATAAA1751.6734703E-1029.5813521
ATGGGGA2350.027.994355
ATGGGAT2600.027.1099115
GCAGAGT14850.026.9171549
Produced by FastQC (version 0.11.2)