FastQCFastQC Report
Sun 14 Apr 2019
SRR6576689_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576689_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162640
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4210.2588539104771274No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2750.1690850959173635No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2650.16293654697491392No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2520.15494343334972946No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2480.15248401377274962No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2330.14326119035907525No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2090.12850467289719628No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2090.12850467289719628No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2070.12727496310870634No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA1920.11805213969503198No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC1750.10759960649286769No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA1750.10759960649286769No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG1740.10698475159862274No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT1690.10391047712739793No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA6350.038.5585751
GTACATG12700.035.5925331
CTAAGAC1800.033.943783
TACATGG13100.033.7245252
GGGATCC702.1770458E-433.570777
CATGGGG3950.032.125954
ACATGGG13400.031.9172843
ATTGCAA600.00415097931.332726
AGTAACC600.00415097931.332723
TAAGACA2100.031.3327184
GGTACCT1551.4988473E-930.3219838
AACGCAG8600.030.057556
ATCAACG8550.028.5842323
TCAACGC8750.028.468014
CAACGCA8800.028.3062635
ACCTAAG1503.592504E-828.2515721
ACCAGAT1704.0217856E-927.64651594
ATTATCC856.791552E-427.6465153
CATGGGT2900.027.5511844
ATGGGAT1904.4019544E-1027.2099915
Produced by FastQC (version 0.11.2)