FastQCFastQC Report
Sun 14 Apr 2019
SRR6576690_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576690_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147189
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4300.292141396435875No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2780.18887280978877496No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2570.17460543926516248No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2570.17460543926516248No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2380.16169686593427499No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2110.14335310383248748No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC2010.1365591178688625No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA1980.13452092207977498No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG1920.1304445305016No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA1920.1304445305016No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT1870.12704753751978748No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT1870.12704753751978748No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC1800.12229174734524999No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG1780.12093295015252498No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC1720.11685655857434998No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG1720.11685655857434998No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG1710.11617715997798748No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA1590.10802437682163749No Hit
GATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGG1590.10802437682163749No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA1570.10666557962891249No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC1530.10394798524346249No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1500.10190978945437498No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7400.041.9222531
TTATAGA608.8000976E-539.1564754
CATGGGT2650.037.2355884
GTACATG12950.034.844471
TACATGG12550.034.8314552
ACATGGG12750.034.273433
TAGTACT550.002712811334.1729244
AACGCAG8350.033.7636646
TAGGACC1309.096766E-932.5299954
ATCAACG9250.032.002483
TCAACGC9200.031.665674
TTCTAAG600.00414768931.3358292
GCCTAGA600.00414768931.3358291
ACCTAAG902.5772973E-531.3358251
CAACGCA9550.030.9971645
GGTACCT1252.3449138E-730.0721728
ATTCAAT1102.9434905E-629.911471
ACGCAGA9450.029.8335027
CCTAAGA953.7282967E-529.6865712
CGCAGAG9500.029.6764878
Produced by FastQC (version 0.11.2)