FastQCFastQC Report
Sun 14 Apr 2019
SRR6576691_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576691_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences238240
Sequences flagged as poor quality0
Sequence length100
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4670.19602081934184018No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3040.12760241773002015No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2680.11249160510409671No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2470.10367696440564138No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA5350.050.0942421
TCAACGC5950.044.2060554
GGTATCA2100.042.5403481
CAACGCA6200.042.4235535
ATCAACG6300.041.758933
AACGCAG6550.040.1566476
GTCTTAT853.9260703E-738.7209361
CCGAACT500.001696073837.6146241
GTACTAA550.002707533834.1951141
TATCAAC7950.033.7041362
CCTACAC1151.1353768E-732.68093
CGCAGAG8350.031.5001228
GTACATG10150.031.4999331
GTCTAGG753.2603586E-431.345521
CATTGGG600.004150783631.3389342
ACGCAGA8550.030.7632797
TATAGTA1551.5043042E-930.3280032
GTAGCGC1252.359211E-730.066433
TAGCGCG1252.3635585E-730.060124
TACATGG10550.029.40812
Produced by FastQC (version 0.11.2)