FastQCFastQC Report
Sun 14 Apr 2019
SRR6576691_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576691_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences238240
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4220.17713230355943585No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3900.16370047011417058No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2580.1082941571524513No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2570.10787441235728676No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA6850.049.5581931
TAGCCCT1350.041.7695664
GGTATCA3050.041.7385641
TCAACGC8100.040.6093034
GGTACCT2050.038.9679538
ATCAACG8450.038.927263
CAACGCA8550.038.471975
ATGGGTA2200.038.4469875
AACGCAG8800.037.9130026
GAGTTAC500.00167307437.719291
TATCAAC9400.035.5004772
CATGGGT3400.034.5520324
CCTATTG550.00271252734.18227894
GGGTACC2350.033.9933177
ACGCAGA9900.033.7004477
CGCAGAG10150.032.8703888
TACATGG11700.032.1371842
GTACATG11750.032.101531
ACATGGG11950.030.6717973
TGGGTAC2800.030.2083476
Produced by FastQC (version 0.11.2)