FastQCFastQC Report
Sun 14 Apr 2019
SRR6576692_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576692_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158834
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5390.33934799853935554No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3930.24742813251570822No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3280.20650490449148168No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2920.18383973204729467No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2870.18069179143004646No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2800.176284674565899No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2760.17376632207210044No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT2520.15865620710930908No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC2470.1555082664920609No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2390.15047156150446378No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2370.1492123852575645No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2300.144805268393417No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA2120.13347268217132352No Hit
GGGCGGGGACGGGCGGTGACTCGCCTCGCGGCGGACCGCCCGCCCGCTCC2070.13032474155407534No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC1980.12465844844302856No Hit
GATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGG1940.12214009594923002No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG1920.12088091970233072No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC1910.1202513315788811No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT1890.11899215533198182No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG1790.11269627409748542No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA1740.10954833348023722No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG1680.10577080473953938No Hit
CGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCC1680.10577080473953938No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC1640.10325245224574084No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA1610.10136368787539193No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATAC250.005228798456.3971633
CGATACC250.005228798456.3971634
TACCGTG352.9305188E-453.7115827
CTACTAT555.118918E-542.927871
TACATGG10950.039.9158022
ACATGGG11050.039.5545733
GTACATG10900.039.4227521
GATATAG853.7852806E-738.8876041
CATGGGT2450.036.447154
CTATAGT550.00271066334.18014
ATGGGTA1800.033.942745
CATGGGC1800.033.942744
GTATCAA4550.033.2101331
CATGGGA5450.031.9066544
CATGGGG4350.031.3317584
TCGCACT300.004157513431.33175730-31
CTGACTA600.004151311731.3317579
TGGGTAC1551.4988473E-930.3210546
ACAATCA650.006139933628.9216258
ATACCTC650.006139933628.9216256
Produced by FastQC (version 0.11.2)