FastQCFastQC Report
Sun 14 Apr 2019
SRR6576694_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576694_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences455818
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11840.2597527960721165No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7220.15839655301019268No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6310.1384324445282986No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT6130.13448349999341844No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG5890.12921824061357823No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5850.12834069738360487No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA5550.1217591231588046No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5530.12132035154381793No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4670.10245317209939053No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4610.1011368572544305No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11350.048.4535831
GTACATG27500.039.312531
ATCAACG13700.038.7655643
ACATGGG27550.038.55453
TCAACGC13900.038.2077874
GTATTGA1003.316927E-837.603291
ATGGGCG1003.3200195E-837.5991635
AACGCAG14100.037.3325046
TACATGG28700.037.1774672
CAACGCA14450.036.753515
CATGGGT6950.034.4884454
ACGCAGA16050.032.7967837
CATGGGA9700.031.009624
ATGGGTA3950.030.9360245
CATGGGG15100.030.1913834
CGCAGAG17750.029.655688
GGTACCT4050.029.0117038
ATGGGAG5350.028.990015
TATCAAC19200.028.1535072
GCAGAGT18800.027.7493849