FastQCFastQC Report
Sun 14 Apr 2019
SRR6576694_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576694_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences455818
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10720.23518158563286223No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7100.15576392332027256No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6360.13952937356576528No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5950.13053455545853826No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5860.12856008319109818No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5490.12044280831384456No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG5350.11737140700893778No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG5260.11539693474149772No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG5010.10991228955416416No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4930.10815720309421742No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4850.10640211663427071No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10850.040.368021
GTACATG26300.036.8888971
GTCTTAA651.3952967E-436.2277071
TACATGG27650.035.018522
ACATGGG28750.034.005663
AACGTTA300.00416146131.33190720-21
ATGGGAG5700.030.510735
CATGGGG13700.030.1917174
ATGGGGA7750.029.7180355
CATGGGA11500.029.4279774
TCAACGC14450.029.2752364
CATATAG650.006089295728.9821661
GGTATCA5850.028.9821661
CCATATA650.00614839728.9249342
TATACTA650.00614839728.9249343
ATCAACG14800.028.5829163
CAACGCA14850.028.4866775
AACGCAG14850.028.4866776
GTATAGG1005.2431285E-528.2576121
CCCGATA856.807179E-427.64883494