FastQCFastQC Report
Sun 14 Apr 2019
SRR6576695_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576695_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences412974
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11090.2685399080813804No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7130.1726500941947919No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6790.16441713037624645No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5580.13511746502201108No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG5480.13269600507538004No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5300.12833737717144422No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA5170.12518947924082388No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5020.12155728932087735No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4660.11284003351300566No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4640.11235574152367947No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC4450.10775496762508051No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT4450.10775496762508051No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4190.10145917176383985No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4130.10000629579586123No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9900.042.2565041
ATGGGTA4500.041.7665335
GTACATG26000.041.761621
ACATGGG26000.041.565733
TACATGG27100.040.0664982
GGTACCT4100.038.965128
CATGGGT7700.038.444194
ATGGGAG4400.035.240515
TGGGTAC5150.032.8455246
GGGTACC5050.032.5654877
CATGGGA10500.032.21994
TCAACGC12950.030.478284
ATCAACG12800.030.4683553
AACGCAG13000.029.9996156
GTACCTG5550.029.631669
CATGGGG13150.029.3000954
CAACGCA13350.029.2131065
GTAAGGC700.00884118526.8499153
ACGCAGA14950.026.4009177
ATAGGCA1101.0186517E-425.6294615
Produced by FastQC (version 0.11.2)