FastQCFastQC Report
Sun 14 Apr 2019
SRR6576696_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576696_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences160188
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3750.23409993257921943No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2240.13983569306065372No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2240.13983569306065372No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT1990.12422903088870577No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA1980.12360476440182785No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA1810.11299223412490322No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA1750.10924663520363574No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG1690.10550103628236822No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGACG250.00522907656.396639
TCTATAC451.6082355E-552.21913
ATGGGAC851.4188117E-1049.761735
TACATGG10550.041.4546852
GTACATG10550.041.0089381
ACATGGG10350.040.8671233
ACTAGGC608.793194E-539.1643263
GTACTAG500.00169295137.6212431
CTAAGAC651.4085614E-436.1516843
CATTATA801.1479871E-535.2699172
ATTATAC550.00271085934.179773
ACAGTAC702.1774136E-433.569428
CATCGTC702.1774136E-433.569429
CATGGGG3950.033.3144654
CATGGGT2400.033.2896774
GTATCAA4750.032.671081
TAGGCAT600.004151609331.331465
CATGGGA5750.031.0590134
ATGGGTG953.7338898E-529.6824385
GTATTAA804.7470053E-429.3915981