FastQCFastQC Report
Sun 14 Apr 2019
SRR6576697_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576697_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145151
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4360.30037684893662464No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1990.1370986076568539No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA1970.13572073220301617No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT1900.13089816811458413No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA1860.12814241720690867No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA1860.12814241720690867No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG1650.11367472494161254No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA1650.11367472494161254No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT1540.10609640994550502No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG1520.1047185344916673No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAACGC200.002153294970.548362
GCAAACG200.002156242770.524041
AAACGCA200.002159193670.499743
CTAAAGC250.005226927356.399794
CACCATA405.660344E-446.999837
CTATAAG450.001009329941.7920231
GGTACCT1051.1241355E-940.285578
GTATCAA5650.039.110681
GGGTACC1101.7735147E-938.4544037
GTACATG9400.036.5124471
ACATGGG9100.036.1537133
CCTATTC651.4074438E-436.1537133
AACGCAG5950.035.5460856
GTCCTAT801.1490067E-535.262021
TGCACAG801.1517259E-535.249875
CATGGGA4750.034.631454
TACATGG9850.034.37892
ATGGGAC1107.617746E-834.181695
TAGAGCA550.00270927734.181694
CTATTCC702.1756912E-433.5713044