FastQCFastQC Report
Sun 14 Apr 2019
SRR6576698_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576698_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences245793
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7280.29618418750737413No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6890.2803171774623362No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5650.22986822244734392No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4980.2026095128827916No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4940.2009821272371467No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4680.19040412054045477No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC4390.17860557460952917No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4220.17168918561553828No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3980.1619248717416688No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3950.16070433250743513No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC3880.15785640762755654No Hit
GGGCGGGGACGGGCGGTGACTCGCCTCGCGGCGGACCGCCCGCCCGCTCC3540.14402362963957477No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3490.14198939758251863No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG3490.14198939758251863No Hit
CTTTTTAACTGCAGCAACTTTAATATACGCTATTGGAGCTGGAATTACCG3310.13466616217711652No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT3290.13385246935429404No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3200.13019085165159303No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG3140.12774977318312564No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG2940.11961284495490107No Hit
ACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACG2920.11879915213207862No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC2880.11717176648643371No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG2710.11025537749244282No Hit
CGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAG2510.10211844926421827No Hit
GATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGG2490.1013047564413958No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAG2480.10089791002998458No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT2470.10049106361857335No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG409.604082E-870.6203161
AAGACTC250.005233802456.3927965
CCCGTTC250.005233802456.3927963
GACTCGT352.9348093E-453.7074247
GTATCAA7050.050.753131
GTACATG16300.039.570481
ACATGGG16350.038.2275353
TACATGG17600.036.5805552
TCAACGC9700.036.3355674
ATCAACG9850.036.2593273
CAACGCA9800.035.4852645
AACGCAG9800.035.4852646
CATGGGG6350.034.782964
TGGGCGA702.1816624E-433.567146
CATGGGT4650.032.3399544
ATGGGGG2350.031.9959145
ATGGGCG600.00415727431.3293325
AATGCGA300.004161285231.32933220-21
ACTAGGC1201.645185E-731.3293323
ATGGGTA3050.030.8157375
Produced by FastQC (version 0.11.2)