FastQCFastQC Report
Sun 14 Apr 2019
SRR6576700_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576700_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences286817
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5360.1868787414971916No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3450.12028575712039384No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3340.11645055906727983No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3170.11052343480337638No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2900.10110976685482381No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7500.050.8626441
GTATATC450.001002662341.862261
ATCAACG10150.037.5046043
TCAACGC10450.036.8776474
GTCCTAA906.071841E-736.629481
CAACGCA10400.036.6030545
AACGCAG10500.035.8068666
ACATGGG17250.035.6901053
TACATGG17400.035.652532
GTACATG17800.035.4535681
ACGCAGA11050.034.0246247
CATGGGG6900.032.6932264
CGCAGAG11500.032.6932268
CATGGGT4050.031.3310094
TATCAAC12450.031.3310092
GGTATCA3800.030.9835761
GCAGAGT13450.027.9533149
ATGGGGG3250.027.4748845
GTCTTAC700.0087386326.9114511
CTAGACA700.00882836326.855154
Produced by FastQC (version 0.11.2)