FastQCFastQC Report
Sun 14 Apr 2019
SRR6576701_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576701_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences257488
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6250.24272975827999752No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3850.14952153110047847No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3310.12854967998508668No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2770.1075778288696949No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2730.10602435841670292No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2710.10524762319020693No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2650.10291741751071895No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2590.10058721183123097No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7000.047.710271
GCTAGAC405.672855E-446.9926223
CTTACAC503.0056599E-546.992623
GTACATG15650.044.1829381
TACATGG16050.043.039972
GGACTAA450.001013271341.7712176
ACATGGG16600.041.047773
ATCAACG8850.037.7002943
AACGCAG9100.036.6645746
TGTATAG651.4117488E-436.148175
TCAACGC9250.036.070014
GTATAGC801.1564083E-535.2444656
CAACGCA9700.034.881125
TATCAAC9900.034.6511232
CATGGGT4250.034.276974
CATGGGA8100.033.6490364
CTTATAG851.7514023E-533.171263
CGCAGAG10300.032.874758
ACGCAGA10400.032.08157
GTATAGT600.00413850431.3588641