FastQCFastQC Report
Sun 14 Apr 2019
SRR6576702_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576702_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences216900
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4370.20147533425541722No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2880.13278008298755187No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2580.11894882434301522No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2390.11018902720147533No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA2350.10834485938220378No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGT301.3689316E-462.6642076
ACCGTCG250.00523114356.3977858
GTATCAA5250.043.0491331
TCAACGC5950.038.7043654
ATCAACG6050.038.0646253
CAACGCA6100.037.7526175
TCCGACC500.001699312437.5985268
GTACATG11150.036.3166731
CATGGGG5050.036.2956054
TACATGG10950.036.119992
AACGCAG6400.035.9829646
GGTATCA2550.035.082931
ACATGGG11150.034.5636673
ACGCAGA7200.031.9848567
GTCTAAT600.0041170331.3899941
CTATAGG600.00415440931.3321044
TGTATAG902.5850019E-531.3321045
GCGGCAC300.00415895131.33210466-67
CTACACG600.00415440931.3321044
TACCGCC300.00415895131.33210468-69
Produced by FastQC (version 0.11.2)