FastQCFastQC Report
Sun 14 Apr 2019
SRR6576703_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576703_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences325206
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7860.2416929576945075No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4860.14944373720042067No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4070.12515144247031113No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3890.11961648924066592No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3880.11930899183901897No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8350.045.6116141
AGGCCGT450.001013126941.7757266
GTACATG18400.038.586561
TACATGG18300.038.283542
ATCAACG10100.037.2258953
ACATGGG18950.036.9533353
AACGCAG10250.036.681136
TCAACGC10550.035.638064
CAACGCA10650.035.3034325
CCCGCTA550.002714811434.180143
TTAGACG352.1845929E-433.5697816-17
CATGGGA8300.032.2755284
GTCATAT600.004148253731.3462561
AATAGAC753.2713605E-431.3317953
TATCAAC12650.030.4789682
ACGCAGA12550.029.958697
GTATAGC804.761922E-429.3871141
CGCAGAG13050.029.1709828
ATGGGAG4300.028.417215
CATGGGG8000.027.0236724
Produced by FastQC (version 0.11.2)