FastQCFastQC Report
Sun 14 Apr 2019
SRR6576703_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576703_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences325206
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7750.23831048627639098No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5330.1638961150778276No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4110.12638143207689895No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3870.119001494437372No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3560.10946907498631638No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3540.10885408018302245No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3480.1070090957731407No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3330.10239663474843638No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8800.044.9857141
TACATGG17900.036.5064622
GTACATG18400.036.3704381
ATCAACG10900.035.7980653
ACATGGG18450.034.908583
AACGCAG11650.034.295276
TCAACGC11700.033.3452074
CAACGCA11800.033.0626225
CATGGGG7150.032.2130054
ACGCAGA12900.030.6077027
CATGGGT5300.029.2670734
CGCAGAG13600.029.0278458
AAACGCA650.00614477328.9259625
CGCGGTA650.00614939228.92151694
ATGGGTA3600.028.7250865
TGGGTAC3150.028.3520366
TATAGCC1005.3027907E-528.2071512
GGGTACC3000.028.2028147
GGTACCT3000.028.1984798
GTGTAGG1554.928188E-827.3645821