FastQCFastQC Report
Sun 14 Apr 2019
SRR6576704_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576704_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences294281
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7420.2521399614654021No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4400.14951695828137052No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3870.13150696103384182No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3810.12946809342091403No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3550.1206330004315603No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3230.10975903982927881No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3150.10704054967870845No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3140.10670073840988714No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3070.10432205952813807No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3070.10432205952813807No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGCTA301.3713862E-462.6493
CCGCTAA405.6776847E-446.9867444
GTATCAA7600.042.047921
CTAGTAC450.001014131241.7663
CGCTAAA500.001702327337.5893945
TGGGTAC3050.033.8920826
AGACCGT702.1846849E-433.561966
GTACATG17650.033.5486451
CATGGGT5900.033.4481934
TACATGG17800.033.2659342
GGGTACC3150.032.816147
ATGGGTA3300.032.748345
AACGCAG10150.032.404656
ATCAACG10050.032.2595563
ACATGGG18100.032.1898163
GGTACCT3250.031.8064128
ACCGTGC600.004161738331.32458
TCAACGC10350.031.3244974
CAACGCA10650.030.8833085
GCAAACG650.006150197228.9198341