FastQCFastQC Report
Sun 14 Apr 2019
SRR6576704_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576704_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences294281
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7260.24670298116426137No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4570.15529374985133257No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3930.1335458286467696No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3850.1308273384961992No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3700.12573016946387977No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3450.11723488774334735No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3100.10534149333460195No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2970.10092394683992512No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8100.047.041081
TACATGG15300.038.0859262
GTACATG15750.037.6328621
ATAATAG757.432225E-637.5944943
ACATGGG15800.037.17813
ATCAACG10150.037.0389143
CAACGCA10600.035.4665035
AACGCAG10500.035.3567286
TCAACGC10650.035.2999954
CATGGGG6100.033.8966754
GATCTAG753.2529535E-431.3607181
CTAGGAC902.5900117E-531.3287473
ATAACGC600.004158978431.3287453
CATGGGT4850.031.005774
ACGCAGA12350.030.0603757
CGCAGAG12450.029.8392078
GTCTATA953.7206395E-529.7101541
TATCAAC12500.029.6996522
ATGGGTA3350.029.4583745
TGGGTAC3300.028.4806776