FastQCFastQC Report
Sun 14 Apr 2019
SRR6576709_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576709_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences440148
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG14720.33443296345774604No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7530.17107881894271926No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA7270.16517171496860147No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT7140.16221816298154257No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6670.15153993656679118No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC5640.12813871697701681No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG5480.1245035760698674No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG5250.11927806101584013No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5110.11609731272208439No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG5040.11450693857520651No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC4990.11337095704172233No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG4910.11155338658814763No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG4770.1083726382943919No Hit
CGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCC4760.10814544198769505No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4750.10791824568099821No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT4660.10587347892072665No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4520.10269273062697092No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10100.052.606291
ATCAACG13500.038.2953
TCAACGC13650.037.8741764
CAACGCA14050.037.1304135
AACGCAG15300.034.4040646
GTACATG25050.033.411261
TACATGG24950.033.3567162
ACGCAGA16100.032.6945467
ACATGGG24800.032.5956733
CGCAGAG16950.031.0549958
ATGGGTA4550.030.9879635
TATCAAC17400.030.2656042
CATGGGT8300.030.0110324
GCAGAGT18300.028.7640539
CATGGGG12850.028.5282174
ATGGGGC2850.028.034145
GGTATCA4050.027.8635731
ATAGTAC1555.070251E-827.28943
GTACCGC700.00883166526.8562326
CGTATAG350.00883637826.85623268-69
Produced by FastQC (version 0.11.2)