FastQCFastQC Report
Sun 14 Apr 2019
SRR6576709_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576709_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences440148
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG12670.28785772058489417No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7850.1783491007570181No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA7000.15903741468778684No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA6830.15517507747394058No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6260.1422248879922208No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5950.1351818024846188No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG5520.12541236129665476No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG5240.11905086470914328No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG5120.11632450902878122No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG4910.11155338658814763No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG4650.10564628261402984No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT4630.10519189000063615No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4520.10269273062697092No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTAGC352.9363047E-453.7113652
GTATCAA8300.051.7094351
ATCAACG10900.039.6675723
TCAACGC11300.038.6793144
CAACGCA11800.037.040365
GTACATG22000.036.01581
AACGCAG12850.035.110936
ACATGGG21900.034.979833
TACATGG22650.034.236552
GGTACCT3850.034.179968
GGTATCA3800.033.5109441
ATGGGTA4150.032.8415875
CTAGGAC1052.0624138E-631.3316273
ACGCAGA14300.030.8934257
TATCAAC14900.029.9648132
CGCAGAG14900.029.6493958
CATGGGT6350.029.604694
GGGTACC4900.027.8148147
CATGGGG13250.027.666424
CATGGGA7450.027.1260414
Produced by FastQC (version 0.11.2)