FastQCFastQC Report
Sun 14 Apr 2019
SRR6576714_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576714_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences422381
Sequences flagged as poor quality0
Sequence length100
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC14760.3494475367026453No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA11600.2746335654302632No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC11230.2658737017053324No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10340.2448026781507691No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC7900.1870349281809551No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG6340.15010144869205766No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT5980.14157833804077363No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5870.13897405423065903No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG5570.13187146202125571No Hit
GTACATGGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAG4820.1141149814977473No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4750.11245770998221984No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGTTGCTGCTGTGTTTGGGACT4630.10961667309845849No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4490.10630213006740359No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4390.10393459933093581No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4380.10369784625728903No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA4280.10133031552082125No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11550.043.5428241
TTAGGCT555.2798707E-542.7238854
TCAACGC12850.039.1330834
ATCAACG12900.038.9814033
CCCTATA757.4304426E-637.6014672
CAACGCA13400.037.5268755
AACGCAG13250.036.8876386
TATACTA651.411652E-436.155262
TACATGG20150.033.822662
GTACATG20600.033.3119771
TATCAAC15750.032.2298282
ACATGGG21150.031.7752553
GGTATCA4250.030.9659161
ACGCAGA16150.030.2638537
TTAGGTA3800.029.6818584
GTCTTAG4000.029.3761481
CTATACT804.7780428E-429.372674
GGGTATA2450.028.7766321
CGCAGAG17100.028.5825278
CATGGGG13000.028.5592734
Produced by FastQC (version 0.11.2)