FastQCFastQC Report
Sun 14 Apr 2019
SRR6576714_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576714_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences422381
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC14120.3342953399892514No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA12410.29381056439565223No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC10550.24977449269735144No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8920.2111837416929265No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC7080.16762117614191926No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6230.1474971648819431No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG6150.14560314029276886No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT5450.12903042513749435No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5040.11932354911797641No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4780.11316796920316018No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4620.10937992002481173No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG4570.10819615465657784No Hit
GTACATGGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAG4470.10582862392011004No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGTTGCTGCTGTGTTTGGGACT4290.10156706859446804No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG459.992338E-441.895991
GTATCAA11750.035.29961
GGTATCA5700.033.902671
TACATGG19300.033.1185042
CTTAGGT3300.032.7530363
GTCTTAG3350.032.359961
TTAGGTA3350.032.2641834
GTACATG19600.032.2235681
GTATATA1201.607732E-731.4219911
GTATATG753.21828E-431.4219881
AGGTATA3450.031.3289956
GTAGGAT600.0041608531.3289933
ACATGGG19950.031.0934373
TAGGTAT3500.030.8814375
GGTATAG3700.030.4822647
ATCAACG13900.030.4274393
CAACGCA14150.029.889855
AACGCAG14200.029.7846076
TCAACGC14300.029.5763234
CATGGGG11800.029.072244
Produced by FastQC (version 0.11.2)