FastQCFastQC Report
Sun 14 Apr 2019
SRR6576715_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576715_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences399405
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG12090.30270026664663685No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7630.19103416331793543No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6660.16674803770608781No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA6370.15948723726543232No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG5990.14997308496388376No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG5790.14496563638412138No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT5690.14246191209424017No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5660.14171079480727583No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5540.13870632565941837No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC5440.13620260136953719No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5320.13319813222167973No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG5100.1276899387839411No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG4470.11191647575768957No Hit
GATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGG4440.1111653584707252No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG4360.10916237903882024No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG4350.10891200660983213No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT4310.10791051689387965No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT4240.10615790989096281No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC4190.1049060477460222No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10800.043.09821
GTACATG25300.038.6536521
TACATGG25900.038.121262
ACATGGG25350.038.0067253
CATGGGA9650.036.5273784
CTCGATA550.002715303634.1807794
CATGGGT6750.032.7249224
ATCAACG13200.031.6884233
CATGGGG10400.031.6336484
CTCGTCG600.004158384631.3323759
CAACGCA13350.031.3323755
CCGACCA1201.6481499E-731.3323759
TAAGGTC600.004158384631.3323755
TCAACGC13500.031.3323734
AACGCAG13750.031.1045026
ATGGGTA5200.029.8260125
ATGGGGG4500.029.243555
TCCGACC1303.3233118E-728.9221928
GGTATCA5400.027.8614641
ATGGGAC2550.027.6462125
Produced by FastQC (version 0.11.2)