FastQCFastQC Report
Sun 14 Apr 2019
SRR6576715_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576715_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences399405
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11780.2949387213480052No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7690.19253639789186414No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6860.1717554862858502No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG6390.15998798212340856No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA6300.1577346302625155No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6280.15723388540453925No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG5710.14296265695221644No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT5480.13720409108548967No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5440.13620260136953719No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5330.13344850465066788No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG5280.13219664250572727No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC5040.1261877042100124No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4830.12092988320126187No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG4110.10290306831411726No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG4040.10115046131120042No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC4040.10115046131120042No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTACGC250.00523412656.398423
GTATCAA10700.048.0033651
GTACATG25600.041.9685331
ACATGGG25750.040.7017753
TACATGG26600.040.2845882
AACGCAG13700.038.4222836
CAACGCA13650.038.2187085
TCAACGC13450.038.0881544
ATCAACG13600.037.6680643
ATGGGAT4050.034.8138435
CATGGGA11800.034.6515774
CATGGGT7200.033.9434974
ATGGGTA4300.033.8827745
TATAGGG702.1828193E-433.5704882
ACGCAGA15950.033.0022137
GGGTACC3600.032.6379787
CATGGGG9600.032.3115964
GGTACCT3700.031.7558698
CGCAGAG16650.031.3324588
TGGGTAC4250.029.8579886
Produced by FastQC (version 0.11.2)