FastQCFastQC Report
Sun 14 Apr 2019
SRR6576716_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576716_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences362404
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11280.3112548426617808No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA7080.1953620820962241No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6960.19205086036577962No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5900.16280173508018675No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5650.1559033564750941No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5640.1556274213308904No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5470.15093652387942738No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG5460.15066058873522367No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG5300.14624562642796438No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC5050.1393472478228717No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4990.13769163695764947No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4710.12996545291994568No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG4250.1172724362865752No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG4200.11589276056555667No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC4170.11506495513294555No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG4150.11451308484453815No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG4090.1128574739793159No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT3940.1087184468162603No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT3830.10568316023001954No Hit
GATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGG3680.10154413306696394No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10600.049.6873661
GTACATG21500.040.6825641
ACATGGG22050.039.208793
TCAACGC13150.038.947084
TACATGG22600.038.702442
AACGCAG13300.038.154556
ATCAACG13450.038.0783733
CAACGCA13500.037.937345
CATGGGT6150.036.6724784
ACGCAGA14800.034.2875337
ATGGGTA3750.033.8303645
CGCAGAG15400.033.256768
GCTCTAA753.2607405E-431.3503631
CATGGGG8650.030.962284
GCAGAGT17250.029.9624829
TGGGTAT1102.9676357E-629.9005766
TATTGTG1154.1952662E-628.600555
TATCAAC18250.028.3441642
CATGGGA9650.028.2406624
GTGCATA856.785424E-427.6620851
Produced by FastQC (version 0.11.2)