FastQCFastQC Report
Sun 14 Apr 2019
SRR6576717_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576717_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences419836
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG12770.30416638878038094No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA8070.19221791366152496No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA8060.19197972541659125No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT7140.17006640688268754No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA7080.1686372774130851No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5840.13910193504130183No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG5640.13433817014262714No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG5530.13171809944835602No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG5520.13147991120342228No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG5210.12409607561047646No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC4890.11647405177259691No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4830.1150449223029945No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG4750.11313941634352462No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG4670.11123391038405472No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT4670.11123391038405472No Hit
GGGCGGGGACGGGCGGTGACTCGCCTCGCGGCGGACCGCCCGCCCGCTCC4650.11075753389418726No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4520.10766108671004869No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC4410.10504101601577759No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC4270.1017063805867053No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCACG200.00216223870.4982154
TAGTATG558.79907E-751.2714277
GTATCAA9300.046.604461
GTACATG21850.040.5348931
TACATGG21800.040.3154952
AACGCAG11100.039.8007936
ATCAACG10950.039.4875873
ACATGGG22050.039.2189643
ATGGGAG4100.038.974625
TCAACGC11250.038.434584
CAACGCA11350.038.095955
GTGTAAT500.001681194437.6888241
ACTAGGC500.001701008537.599053
TACGTGT757.4336203E-637.5990454
TTAGGTA1302.2919266E-1036.152934
TAGGTAT1353.43789E-1034.813935
CATGGGA8650.033.143674
GTATAGT1457.421477E-1032.4129758
GGTATCA4350.031.4073561
GTCTTAG2550.031.4073541
Produced by FastQC (version 0.11.2)