FastQCFastQC Report
Sun 14 Apr 2019
SRR6576718_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576718_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences382590
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11990.31339031339031337No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA8520.22269269975691994No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA7280.19028202514441045No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT6600.17250842938916333No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG5980.1563030920829086No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5970.1560417156747432No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG5950.1555189628584124No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG5770.15081418751143522No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5750.15029143469510442No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG5310.13879087273582685No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC5000.1306882040826995No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4630.12101727698057972No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4520.11814213649076034No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT4520.11814213649076034No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG4430.11578974881727175No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG4400.11500561959277555No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC4290.11213047910295616No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA4040.10559606889882119No Hit
GGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT3940.10298230481716719No Hit
GGGCGGGGACGGGCGGTGACTCGCCTCGCGGCGGACCGCCCGCCCGCTCC3910.102198175592671No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT3830.10010716432734781No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTATG352.9373888E-453.7055447
ATAGTAT503.0085244E-546.9923556
TACATGG21900.041.0002752
GTATCAA9350.040.7739071
GTACATG22100.040.464131
ACATGGG22950.038.9043433
GATCTAG801.1455311E-535.299631
AATAGTA702.1843755E-433.5659645
CATGGGG12250.032.990554
GTCTTAG1755.456968E-1232.273951
AACGCAG11900.031.5914976
GTTCTAA600.004129006531.3774491
GATAATA600.004152875431.3405252
GTATATC753.274611E-431.3282383
TAATAGT902.5923182E-531.3282344
ATCAACG12400.030.3176483
CATGGGA9000.030.283964
ATGGGAG4500.030.283965
TCAACGC12600.029.8364144
CAACGCA12950.029.39295
Produced by FastQC (version 0.11.2)