FastQCFastQC Report
Sun 14 Apr 2019
SRR6576719_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576719_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences352969
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10090.28586079797375974No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6840.19378472330431284No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5570.15780422643348282No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4910.13910570050061052No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4850.13740583450671306No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC4770.13513934651484974No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4730.1340061025189181No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG4320.12239035156061864No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4250.12040717456773824No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4180.11842399757485784No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4120.11672413158096036No Hit
GATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGG4090.11587419858401161No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG4090.11587419858401161No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC4030.11417433259011416No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA3850.10907473460842171No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3830.10850811261045587No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG3630.1028418926307976No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC3570.10114202663690011No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT3530.10000878264096848No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8850.040.897711
GTACATG21850.040.6594851
ACATGGG22100.038.4925163
TACATGG22900.038.3846552
ATGGGTA4250.037.599325
CATGGGT5850.036.9565964
ATCAACG10750.031.9157033
TCAACGC10850.031.621554
GGGTACC4200.031.3327667
AACGCAG11200.030.6333756
CAACGCA11400.030.0959475
TGGGTAC4300.029.5110936
CATGGGG8950.029.4072914
GGTACCT4500.029.2439168
CATGGGA11150.029.0846754
ATGTGCG650.00614901528.9225547
ACGCAGA12100.028.3548587
CGCAGAG12150.028.2381738
ATGGGAT2950.027.0842555
GTACCTG5200.025.3072349
Produced by FastQC (version 0.11.2)