FastQCFastQC Report
Sun 14 Apr 2019
SRR6576720_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576720_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences324884
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10670.3284249147387991No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6710.2065352556604819No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5520.1699067975031088No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5320.163750754115315No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC4720.14528262395193361No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4710.14497482178254392No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4520.13912658056413982No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4130.12712229595794192No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC4080.12558328511099345No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4050.1246598786028244No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3970.12219746124770686No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3890.11973504389258935No Hit
GATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGG3850.11850383521503059No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3840.11819603304564091No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG3720.11450240701296463No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG3610.11111658314967804No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA3590.11050097881089865No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC3540.1089619679639502No Hit
ACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACG3460.10649955060883269No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT3440.1058839462700533No Hit
AGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGG3400.10465273759249455No Hit
GGGCGGGGACGGGCGGTGACTCGCCTCGCGGCGGACCGCCCGCCCGCTCC3300.10157471589859765No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAAA200.002163868970.4811865
ACATGGG21350.039.1745533
GTACATG22350.038.689141
TACATGG22550.037.9291952
CATGGGC3100.034.861664
GTATCAA10300.033.7631761
TAGTCGG352.1873869E-433.5624740-41
CATGGGT6400.031.5696964
CATGGGG6700.031.5587394
GTCTTAC600.004158928531.3297941
CATATAG600.004162063431.324973
AATAACC953.7469712E-529.6808572
GTGCTAA804.776581E-429.3716811
CATGGGA11000.029.0467914
ACATATA1005.3160387E-528.1968142
GGGTATG856.8161206E-427.6396797
ATGGGAC2400.027.409355
TCAACGC12200.027.3451584
ATGGGTA4650.027.283045
AACGCAG12100.027.1828256
Produced by FastQC (version 0.11.2)