FastQCFastQC Report
Sun 14 Apr 2019
SRR6576720_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576720_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences324884
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10570.32534689304490216No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7310.22500338582386328No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6760.20807426650743033No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA6630.20407283830536438No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5610.172677017027616No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4890.15051526083155836No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4700.14466701961315423No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4620.14220460225803672No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC4580.14097339358047797No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4390.13512515236207384No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG4390.13512515236207384No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4340.13358614151512538No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT4180.12866130680489035No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG3880.11942724172319967No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC3780.11634922002930277No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC3630.11173218748845742No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3560.10957757230272958No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG3550.1092697701333399No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT3510.10803856145578114No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG3360.10342152891493579No Hit
GGGCGGGGACGGGCGGTGACTCGCCTCGCGGCGGACCGCCCGCCCGCTCC3330.10249812240676673No Hit
GATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGG3270.1006513093904286No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT3250.10003570505164922No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGTAC250.005236061356.3907663
ACGCGTA352.936743E-453.705496
TACGCGT405.6752504E-446.9923065
GTATCAA9500.040.6243061
GTACATG22150.038.2467421
ACATGGG21900.038.1946563
TTATACG500.001701598837.5938452
ATACGCG500.001701598837.5938454
TACATGG22900.036.9371832
ATCAACG11450.033.2434653
AACGCAG11650.032.672766
CATGGGA8950.032.0282754
TCAACGC11950.031.8525264
GTGTTAC600.004128710431.3764951
ATGGGAT2850.031.3282035
TAAGCCT753.2735534E-431.3282034
GGTACCT4550.031.00788
CAACGCA12400.030.6965855
ACTGTGC1551.5006663E-930.3409658
ATGGGTA5150.030.1115745
Produced by FastQC (version 0.11.2)