FastQCFastQC Report
Sun 14 Apr 2019
SRR6576721_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576721_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences375050
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5880.15677909612051727No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5120.13651513131582457No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4320.11518464204772698No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10500.043.561341
GTACATG23250.037.114781
TACATGG23700.036.5016172
ACATGGG23850.036.0749173
AACGCAG13200.034.5493976
ATCAACG13300.033.9361233
CAACGCA13500.033.7816285
TCAACGC13400.033.682874
CATGGGA12300.030.961544
TATCAAC15150.030.1024442
ACGCAGA15800.028.8525077
CATGGGG9550.028.554024
GTAATAC1005.302045E-528.2094023
CGCAGAG16350.027.8819338
GTCATAA856.6784595E-427.7376631
GTATTAT909.32582E-426.196681
TAGACCT1301.0824784E-525.3161324
GCAGAGT17950.025.1348159
ATGGGAG5850.024.9142865
AAAGGCG1551.5430269E-624.25644794
Produced by FastQC (version 0.11.2)