FastQCFastQC Report
Sun 14 Apr 2019
SRR6576722_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576722_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences339310
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6250.1841973416639651No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3620.10668710029176859No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3490.1028557955851581No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTAA250.00523663856.389744
GTATCAA9500.043.027941
AGCGGTC555.2854535E-542.71320794
TTACGCG308.837386E-539.15377480-81
GTACATG20800.034.3348461
CCGAACT550.002718707834.1705671
TACATGG21200.033.027012
GTATAAT1001.4109082E-632.8891681
ACATGGG21300.032.6513373
AACGCAG12500.032.330126
ATCAACG12400.031.8329163
CAACGCA12750.031.3276355
CGCCGGT902.5918E-531.3276337
TCAACGC12700.031.0809614
ATGGGAG5150.030.1110275
CATGGGA12100.029.9036524
TGCCCGC1102.9679304E-629.8992441
CTTATGG650.00615360528.9178143
ACGCAGA14250.028.3597537
CGCAGAG14350.028.1621258
Produced by FastQC (version 0.11.2)