FastQCFastQC Report
Sun 14 Apr 2019
SRR6576723_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576723_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124066
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2080.16765270098173551No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG1260.10155884771008979No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAACT200.002155044370.527821
TCAGCGG250.005225245456.3995175
TAACGCC405.657285E-446.9995964
GTATCAA4800.043.1003381
CATGGGT1450.042.137574
GTATAAT450.001008496341.7942661
GTACATG11400.041.244341
TACATGG11450.041.0642362
ACATGGG12200.038.1390153
GAGTGCA500.001696253237.5996789
GGAGATC757.3905503E-637.5996788
CATGGGA7750.033.3545534
CATGGGG2700.033.073794
ATGGGAG2900.032.4135175
TCCTACA600.00413881331.3457012
ATGGGCG600.00414699331.3330635
ATGGGAC1807.2759576E-1231.3330635
AACGCAG6600.031.3330636
GTATACA600.00414699331.3330634
ATCAACG6850.029.5033993
Produced by FastQC (version 0.11.2)