FastQCFastQC Report
Sun 14 Apr 2019
SRR6576723_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576723_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124066
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3460.27888381990231004No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1760.14185997775377623No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG1450.11687327712669063No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA1340.10800702851707963No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA1280.10317089291183724No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGAT1850.050.808855
GTACAAA405.557583E-447.1692961
CAGTATA450.001010649441.776179
GTATCAA4950.037.1636851
TACATGG10900.037.081142
GTACATG11000.036.877811
TGGGATG1152.733941E-936.781196
ACATGGG11200.036.0878943
CATGGGC1750.034.9129374
CATGGGA6750.032.7246674
GTGTAGG600.004074424531.44621
GGGATGG753.2595926E-431.3321277
CATGGGT1551.4915713E-930.321414
ATGGGAC1252.3353277E-730.0788365
GGATATG650.006026433329.0272581
GTGTAAA650.006026433329.0272581
ACAATAG650.00613447328.921968
CAGTAGA650.00613447328.921964
CATGGGG2700.027.8507774
ATGGGGG856.783157E-427.6459925
Produced by FastQC (version 0.11.2)