FastQCFastQC Report
Sun 14 Apr 2019
SRR6576724_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576724_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119812
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2520.21032951624211263No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG1300.10850332187093113No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA1220.10182619437118152No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTGA250.005222605556.4055141
TAGGGGT250.005231246456.3819734
GCGCAAA555.2646195E-542.71361594
TAGGCAG555.2646195E-542.7136155
TGCCCGC450.001009831141.7818641
GTATCAA6500.039.049971
ATGGGAC1101.7735147E-938.4422535
GTACATG13450.038.092941
ATGGGAG2600.037.94945
TACATGG13550.037.8118132
ACATGGG13050.037.8043
CATGGGA8050.036.770854
CATGGGT2100.035.7980774
CTACAAC550.002711597134.170893
ATGGGAT2350.033.9891325
GTATTAA600.004144267631.3363951
GTACAAG600.004144267631.3363951
AACGCAG8150.029.401646
ACAAGAC650.00614207528.9138323
CATGGGG3300.028.4757444
Produced by FastQC (version 0.11.2)