FastQCFastQC Report
Sun 14 Apr 2019
SRR6576725_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576725_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences394230
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC13430.34066407934454507No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA10790.2736980950206732No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC10110.2564492808766456No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9460.2399614438272075No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC6720.17045886918803743No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG5930.15041980569718186No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5380.13646855896304189No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG5230.1326636734900946No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT5110.12961976511173678No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4770.120995358039723No Hit
GTACATGGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAG4110.10425386195875505No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4080.1034928848641656No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGTTGCTGCTGTGTTTGGGACT4010.10171727164345686No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA4010.10171727164345686No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTAA255.5559627E-575.196954
CCCGCTA301.3703233E-462.6641273
CGCTAAA352.9357255E-453.7121165
ATAGTAC503.0065197E-546.9980963
GTATCAA9400.041.5404741
ATCAACG10200.037.3220183
CAACGCA10550.036.0838475
TCAACGC10600.035.913644
TACATGG20250.035.3134162
GTACATG20550.035.255761
TAGTATG1204.1945896E-935.2485737
AACGCAG11000.035.0349466
ACATGGG20500.034.388853
GGTATCA4000.034.108231
CATGGGG12050.031.5920814
GGTAATC1351.322951E-831.3320668
GTTACAC753.2724452E-431.3320643
TATCAAC12500.030.8620682
ATAATAG1701.2187229E-1030.4105363
GATAATA1401.866465E-830.2437532
Produced by FastQC (version 0.11.2)