FastQCFastQC Report
Sun 14 Apr 2019
SRR6576727_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576727_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences334082
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7360.22030519453307867No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5210.15594973689094294No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4660.13948671284295472No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4230.12661562131452758No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3940.11793511772558833No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3710.11105058039642961No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10100.052.1266981
ATCAACG13100.039.812573
AACGCAG13300.039.2138866
TCAACGC13400.038.921244
TAGTACA853.9536644E-738.6943554
GCCGCAA500.001702788937.5888023
CAACGCA14000.036.9175765
TATCAAC14650.035.6163142
GCGTTAT550.00271224834.1869931
GTACATG23000.033.7224921
GTATAGA851.7492603E-533.181491
ACATGGG23150.033.083023
TACATGG23250.032.9555242
ACGCAGA16450.031.7048427
CGCAGAG16700.031.2302198
CATGGGG8750.031.1450084
CATGGGT5100.030.402714
CAATTCG404.7871043E-429.36625378-79
GTCTTAT650.006143457328.9274561
GTCCAAT856.7998754E-427.6512431
Produced by FastQC (version 0.11.2)