FastQCFastQC Report
Sun 14 Apr 2019
SRR6576728_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576728_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences322553
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6050.18756607441257717No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4280.13269137165055045No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4240.1314512653734425No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3370.10447895384634463No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3230.1001385818764668No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8550.040.226771
GTACATG19200.036.563141
ACATGGG19250.035.889433
TACATGG19300.035.5529372
CATGGGT4300.033.8823244
AACGCAG10350.033.148396
ATCAACG10550.032.074513
CAACGCA10750.031.4777745
TCAACGC10850.031.1876564
GGCTAGA953.6821002E-529.7567881
CATGGGA11100.028.7916074
ACGCAGA12100.027.5773757
CATGGGG7300.027.0399824
CGATTAC350.00883497826.85603380-81
CGCAGAG12700.026.2745088
GTATAAA1458.436382E-725.9944381
TATCAAC13350.025.6993162
ATGGGTA3000.025.0656325
GTATATA1701.209155E-724.9431921
GAGGATA950.001302865924.7358239
Produced by FastQC (version 0.11.2)